Journal: Cell

Article Title: Deconstruction of corticospinal circuits for goal-directed motor skills

doi: 10.1016/j.cell.2017.08.014

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken monoclonal anti-GFP Abcam Cat#ab1397 0 Rabbit polyclonal anti-RFP Abcam Cat#ab3477 1 Mouse monoclonal anti-NeuN Millipore Cat#MAB37 7 Rabbit polyclonal anti-PKC gamma Santa Cruz Cat#sc211 Goat polyclonal cholinergic acetyltransferase Millipore Cat#AB144 P Biological Samples N/A N/A N/A Chemicals, Peptides, and Recombinant Proteins Diphtheria toxin Sigma Cat# D0564 Green Retrobeads™ Lumafluor Cat#N/A Clozapine N-oxide Enzo Life Sciences Cat#BML-NS105-0025 Critical Commercial Assays N/A N/A N/A Deposited Data N/A N/A N/A Experimental Models: Cell Lines N/A N/A N/A Experimental Models: Organisms/Strains Mouse/C57Bl/6 Charles River Strain code#027 Mouse/Emx1Cre The Jackson Laboratory Jax#5628 Mouse/Rosa-LSL-ChR2EYFP The Jackson Laboratory Jax#12569 Mouse/ChAT-Cre The Jackson Laboratory Jax#028861 Mouse/Thy1-ChR2EYFP The Jackson Laboratory Jax#7612 Recombinant DNA AAV-GFP This paper Cat#N/A AAV-FLEX-DTR This paper Cat#N/A AAV-FLEX-PLAP This paper Cat#N/A AAV-syn-FLEX-GCaMP6 UPenn Cat#PV2821 AAV-syn-FLEX-hM4Di-mCitrine Addgene Cat#44362 AAV-CAG-FLEX-GFP Addgene Cat#28304 AAV-EF1α-FLEX-tdTomato Addgene Cat#50462 AAV-tdTomato-P2A-SypEGFP This paper Cat#N/A AAV-FLEX-tdTomato-P2A-SypEGFP Addgene Cat# 51509 AAV-CAG-FLEX-oG-WPRE-SV40pA Addgene Cat#74292 Lenti-HiRet-GFP This paper Cat#N/A Lenti-HiRet-mCherry This paper Cat#N/A Lenti-HiRet-Cre This paper Cat#N/A Lenti-HiRet-FLEX-DTR This paper Cat#N/A Rabies-ΔG-mCherry Kim et al., 2016 Cat#N/A Rabies-ΔG-GFP Kim et al., 2016 Cat#N/A Sequence-Based Reagents N/A N/A N/A Software and Algorithms Matlab 2016b Mathworks https://www.mathworks.com/ pClamp 10 Molecular Devices https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software ImageJ2 NIH https://imagej.nih.gov/ij/index.html ImageJ Stabilizer N/A http://www.cs.cmu.edu/~kangli/code/Image_Stabilizer.html Imaris 8.0 Bitplane http://www.bitplane.com/imaris/imaris Matlab CellSort toolbox Matlab central https://www.mathworks.com/matlabcentral/fileexchange/25405-emukamel-cellsort Matlab fastICA toolbox Matlab central https://www.mathworks.com/matlabcentral/linkexchange/links/2115-the-fastica-package-for-matlab Inscopix nVista 2.0.32 Inscopix https://www.inscopix.com/ Mosaic Inscopix https://www.inscopix.com/ Other N/A N/A N/A Open in a separate window KEY RESOURCE TABLE

Techniques: Recombinant, Sequencing, Software

Journal: Cell

Article Title: Neuronal Representation of Social Information in the Medial Amygdala of Awake Behaving Mice

doi: 10.1016/j.cell.2017.10.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cellsort MATLAB algorithm for extracting cellular Ca 2+ signals , Ziv et al., 2013 , N/A.

Techniques: Virus, Plasmid Preparation, Recombinant, RNAscope, Multiplex Assay, Software, Imaging, Microscopy

Journal: Cell

Article Title: Neuronal Representation of Social Information in the Medial Amygdala of Awake Behaving Mice

doi: 10.1016/j.cell.2017.10.015

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Flex. mCherry Penn Vector Core N/A Chemicals, Peptides, and Recombinant Proteins L-368,899 hydrochloride TOCRIS 26-411-0 Critical Commercial Assays RNAscope Fluorescent Multiplex Reagent Kit Advanced Cell Diagnostics 320850 RNAscope probe Mm-Slc32a1 Advanced Cell Diagnostics 319191 RNAscope Probe Mm-Slc17a6-C2 Advanced Cell Diagnostics 319171-C2 RNAscope Probe Mm-Oxtr-C3 Advanced Cell Diagnostics 402651-C3 Experimental Models: Organisms/Strains Mouse: wild type C57BL/6J Jackson Laboratory JAX:000664 Mouse: Vgat-ires-Cre Watabe-Uchida et al., 2012 N/A Mouse: Vglut2-ires-Cre Krashes et al., 2014 N/A Software and Algorithms ImageJ NIH https://imagej.nih.gov/ij/ Cellprofiler Broad Institute http://cellprofiler.org/ MATLAB MathWorks https://www.mathworks.com/products/matlab.html Ca 2+ signal acquision nVista https://www.inscopix.com/ Cellsort MATLAB algorithm for extracting cellular Ca 2+ signals Ziv et al., 2013 N/A Registration of cell identities across imaging sessions Grewe et al., 2017 N/A Fast nonnegative deconvolution Vogelstein et al., 2010 N/A Other 0.85-mm custom GRIN lens Grintech http://www.grintech.de/ Miniatureized microscope camera Inscopix https://www.inscopix.com/ Open in a separate window KEY RESOURCES TABLE

Techniques: Virus, Plasmid Preparation, Recombinant, Multiplex Assay, Software, Imaging, Microscopy

Journal: Neuron

Article Title: Estimation of Current and Future Physiological States in Insular Cortex

doi: 10.1016/j.neuron.2019.12.027

Figure Lengend Snippet: Key Resources Table

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Chicken anti-GFP Invitrogen Cat #: A10262; RRID: AB_2534023 Rat anti-mCherry Invitrogen Cat #: {"type":"entrez-nucleotide","attrs":{"text":"M11217","term_id":"333925"}} M11217 ; RRID: AB_2536611 Bacterial and Virus Strains AAV1-hSyn-GCaMP6f Penn Vector Core Addgene 100837 AAV1-hSyn-GCaMP6s Penn Vector Core Addgene 100843 AAV8-hSyn-DIO-hM3Dq-mCherry UNC Vector Core Addgene 44361 AAV8-hSyn-DIO-hM4Di-mCherry UNC Vector Core Addgene 44362 AAV1-DIO-ChR2(H134R)-YFP Penn Vector Core Addgene 20298 AAV8-FLEX-TVA-mCherry UNC Vector Core Addgene 38044 SADΔG–EGFP (EnvA) rabies Salk Gene Transfer Targeting and Therapeutics Core Addgene 32635 AAV8-CaMKII-hM3Dq-mCherry Addgene Addgene 50476 Chemicals, Peptides, and Recombinant Proteins Clozapine-N-Oxide (CNO) NIMH Drug Supply Program Cat# C-929 Compound 21 (C21) Tocris 6422 CNQX Tocris Cat# 0190 D-AP5 Tocris Cat# 0106 Kynurenic Acid Sigma-Aldrich Cat# K3375–5G Picrotoxin Tocris Cat# 1128 Bicuculline Tocris Cat# 2503 TTX Tocris Cat# 1069 4-AP Sigma-Aldrich Cat# A78403 Cholera Toxin B Subunit B (Recombinant), Alexa Fluor 555 Conjugate Thermo Fisher Cat# 34776 Experimental Models: Organisms/Strains Mouse: C57BL/6J The Jackson Laboratory RRID: IMSR_JAX:000664 Mouse: Nos1-Cre knockin (B6.129-Nos1tm1(cre)Mgmj/J) The Jackson Laboratory Strain# 017526; RRID: IMSR_JAX:017526 Mouse: vGlut2-ires-Cre (B6J.129S6(FVB)-Slc17a6tm2(cre)Lowl/MwarJ) The Jackson Laboratory Strain# 028863; RRID:IMSR_JAX:028863 Software and Algorithms MATLAB R2015b, 2019a Mathworks https://www.mathworks.com/products/matlab.html ; RRID: SCR_001622 MonkeyLogic Asaad and Eskandar, 2008 http://www.brown.edu/Research/monkeylogic/ MonkeyLogic2 NIMH https://monkeylogic.nimh.nih.gov/ Scanbox Neurolabware https://scanbox.org/ PCA/ICA neuron identification Mukamel et al., 2009 https://github.com/mukamel-lab/CellSort Glmnet Friedman et al., 2010 https://web.stanford.edu/~hastie/glmnet_matlab/ ImageJ 1.49t NIH https://imagej.nih.gov/ij/ ; RRID: SCR_003070 Open in a separate window Key Resources Table InsCtx ongoing activity patterns reflect current bodily state, not behavioral state Hypothalamic hunger/thirst neurons gate InsCtx responses to food/water cues Hypothalamic hunger/thirst neurons do not gate InsCtx ongoing activity Food/water cues drive transient “simulations” of future bodily states in InsCtx

Techniques: Plasmid Preparation, Recombinant, Knock-In, Software